An Introduction to Peptide Synthesis and the Advantages of SPPS

For the learners of immunology, understanding peptide synthesis may be required. This can be usually defined as a process that helps in the formation of polypeptide sequences, says Kanury Rao. There are certain advantages associated with this process. In this reading, we will be knowing more about the process and its advantages.

Defining Peptide Synthesis

Peptide synthesis can be looked at as a process in which polypeptide sequences are made. These sequences are usually short. They are made when one amino acid is added at once. Through this process, certain sequences can be made. The sequences formed can indicate epitopes for specific protein domains. Moieties like phosphate groups may be unable to modify these domains.

Kanury Rao explains that animals are injected with short peptide sequences when they have been formed. This helps in producing antibodies to fight a polypeptide.

Advantages of SPPS or Solid Phase Peptide Synthesis

Let us now look at a few advantages of SPPS. Mainly, these include the removal of compounds which are soluble as well as controlling the reaction order.

Doing away with Certain Soluble Compounds

A solid phase method can be used for the formation of polypeptides. In such polypeptides, polymer substrate and amino acids get connected. Now, anything which gets connected to this polymer substrate is not required as it gets insoluble. With this, every molecule which is soluble can be detached, explains Mr. Rao.

Controlling the Reaction Order

Amino acids with free forms can be changed via certain chemical groups. These groups help in protecting these acids and prevent reactions between them and the existing molecules. In this process, several chemical groups can be utilized. These groups can be done away with through certain types of chemical treatments. Kanur Rao says that this helps a scientist in checking or limiting the amino acids that may react in an order.

Conclusion

Peptide synthesis is an important process. Based on the explanation provided by Mr. Rao, when it is injected into an animal, antibodies can be made. There are also certain advantages connected to this process. Mainly, it is much helpful in controlling the order of amino acids that cause reactions.